The establishment of the Glebe-St. James United Church is the result of the amalgamation of two separate church congregations. As part of an 1837 Crown Land grant, the boundaries of the Glebe were allocated to the congregations and clergymen of the Church of Scotland. The St. Andrew Presbyterian Church first began their church mission in 1894 with the establishment of a Sunday School located on Third Avenue. The present day Glebe-St. James United Church, initially occupied by the St. Andrews Church in 1905, was erected on the corner of First Avenue and Lyon Street South in 1904 by the architect John William Hurrell Watts. In parallel, the St. James congregation instituted a Sunday school in 1908 and commenced the establishment of their church on Third Avenue in 1913, now occupied as the Glebe Community Centre and a designated heritage property under the Ontario Heritage Act. By 1971, the two church congregations, St. Andrews (Glebe) United and St. James United, joined together to form the present-day parish in the former St. Andrew Presbyterian Church. Throughout its history, the church has undergone multiple alterations, including an addition of the Glebe Montessori School in 1995.